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Stains

Offspring Biosciences is set up to assist your projects with co-staining of multiple biomarkers in tissue sections using a range of techniques, including up to 5-plexed immunohistofluorescence (IHF), 3-plexed chromogenic immunohistochemistry (IHC) and 2-plexed in situ hybridization histochemistry (ISH). We perform these analyses with pre-established panels for multiplexed biomarker staining or by custom build to flexibly adapt their design to your specific needs.

Listed below are examples of the multiplexed analyses we can run for you:

Preestablished Mouse panels

CD8 and NK cell Activity

4-plexed IHF Assay
CD8 Granzyme B
NKp46 Ki67

See our pamphlet

CD4 cell Activity

4-plexed IHF Assay
CD4 PD-1
Tim-3 FoxP3

See our pamphlet

Tumor Phenotype and Activity

4-plexed IHF Assay
Epithelial  Vimentin
Ki-67 Activity Marker

See our pamphlet

Parkinson's Disease Pathology

2-plexed IHF Assay
Alpha-synuclein/pSer129
PGP 9.5

Preestablished Human panels

Alzheimer's Disease Pathology

2-plexed IHF Assay
Amyloid Beta
Phoshporylated-Tau

Macrophage Subsets

3-plexed IHF Assay
CD68 CD163
empty/available space

Pancreatic Islet

3-plexed IHF Assay
Insulin Ki-67
Secretogranin II

Pancreatic Islet

3-plexed IHF Assay
Insulin BrdU
Pan-Islet Cell Marker

Custom build of multiplexed staining panels

Offspring offers custom design and construction of optimized IHF and IHC assays for simultaneous detection of 3-5 biomarkers in tissue sections, flexibly targeting your project-specific needs. We build these assays, using well selected fluorophors or chromogens and the highly sensitive, sequential TSA-based staining technique. The assays are established, optimized and validated to specifically measure each marker without cross-talk between each individual assay according to strict guidelines and criterias. These assays are designed to be run with high precision, consistency and efficacy on the Ventana Discovery Ultra platform.

Offspring also offers services for multiplexed staining of immune cells in tissues sections from human tissues, using kit reagents from:

Pre-established and flexibly adapted staining panels. 4-5-plexed IHF assays directed against various combinations of human immune cell markers. Hapten anti-hapten based detection technique

HI-1A HI-2A HI-3A HI-4A HI-5A HI-6A
CD3 CD68 CD4 CD8 CD8 CD3
CD4 CD163 CD8 CD68 CD68 CD4
CD8 PD-L1 FoxP3 PD-L1 PD1 CD8
CD20 Pan-Cytokeratin PD-1 Pan-Cytokeratin PD-L1 FoxP3

Pre-established staining panels for analysis of various immune cell populations in human tissue sections. 4-plexed IHF assays. Sensitive rolling circle-based detection.

Panel I/O
PD1
Panel I/O
T-Reg
Panel I/O
MDSC
Panel I/O
PD-1
Panel I/O
T-Act
Panel I/O
APC
CD8 CD4 CD11b CD3 CD3 CD11c
CD68 CD8 CD14 CD45RO GrzB CD20
PD-L1 FoxP3 CD15 PD-1 Ki-67 CD68/CD163
PanCytokeratin/
SOX10
PanCytokeratin HLA-DR PanCytokeratin
/SOX10
PanCytokeratin/
SOX10
MHCII