Stains

Offspring Biosciences is set up to assist your projects with co-staining of multiple biomarkers in tissue sections using a range of techniques, including up to 5-plexed immunohistofluorescence (IHF), 3-plexed chromogenic immunohistochemistry (IHC) and 2-plexed in situ hybridization histochemistry (ISH). We perform these analyses with pre-established panels for multiplexed biomarker staining or by custom build to flexibly adapt their design to your specific needs.
Listed below are examples of the multiplexed analyses we can run for you:
Preestablished Mouse panels
Tumor Phenotype and Activity
4-plexed IHF Assay | |
Epithelial | Vimentin |
Ki-67 | Activity Marker |
Preestablished Human panels
Custom build of multiplexed staining panels
Offspring offers custom design and construction of optimized IHF and IHC assays for simultaneous detection of 3-5 biomarkers in tissue sections, flexibly targeting your project-specific needs. We build these assays, using well selected fluorophors or chromogens and the highly sensitive, sequential TSA-based staining technique. The assays are established, optimized and validated to specifically measure each marker without cross-talk between each individual assay according to strict guidelines and criterias. These assays are designed to be run with high precision, consistency and efficacy on the Ventana Discovery Ultra platform.
Offspring also offers services for multiplexed staining of immune cells in tissues sections from human tissues, using kit reagents from:
Pre-established and flexibly adapted staining panels. 4-5-plexed IHF assays directed against various combinations of human immune cell markers. Hapten anti-hapten based detection technique
HI-1A | HI-2A | HI-3A | HI-4A | HI-5A | HI-6A |
CD3 | CD68 | CD4 | CD8 | CD8 | CD3 |
CD4 | CD163 | CD8 | CD68 | CD68 | CD4 |
CD8 | PD-L1 | FoxP3 | PD-L1 | PD1 | CD8 |
CD20 | Pan-Cytokeratin | PD-1 | Pan-Cytokeratin | PD-L1 | FoxP3 |
Pre-established staining panels for analysis of various immune cell populations in human tissue sections. 4-plexed IHF assays. Sensitive rolling circle-based detection.
Panel I/O PD1 |
Panel I/O T-Reg |
Panel I/O MDSC |
Panel I/O PD-1 |
Panel I/O T-Act |
Panel I/O APC |
CD8 | CD4 | CD11b | CD3 | CD3 | CD11c |
CD68 | CD8 | CD14 | CD45RO | GrzB | CD20 |
PD-L1 | FoxP3 | CD15 | PD-1 | Ki-67 | CD68/CD163 |
PanCytokeratin/ SOX10 |
PanCytokeratin | HLA-DR | PanCytokeratin /SOX10 |
PanCytokeratin/ SOX10 |
MHCII |