Stains

Offspring Biosciences is set up to assist your projects with co-staining of multiple biomarkers in tissue sections using a range of techniques, including up to 5-plexed immunohistofluorescence (IHF), 3-plexed chromogenic immunohistochemistry (IHC) and 2-plexed in situ hybridization histochemistry (ISH). We perform these analyses with pre-established panels for multiplexed biomarker staining or by custom build to flexibly adapt their design to your specific needs.

Listed below are examples of the multiplexed analyses we can run for you:

Preestablished Mouse panels

CD8 and NK cell Activity

4-plexed IHF Assay
CD8	Granzyme B
NKp46	Ki67

See our pamphlet

CD4 cell Activity

4-plexed IHF Assay
CD4	PD-1
Tim-3	FoxP3

See our pamphlet

Tumor Phenotype and Activity

4-plexed IHF Assay
Epithelial	Vimentin
Ki-67	Activity Marker

See our pamphlet

Parkinson's Disease Pathology

2-plexed IHF Assay

Alpha-synuclein/pSer129

PGP 9.5

Preestablished Human panels

Alzheimer's Disease Pathology

2-plexed IHF Assay

Amyloid Beta

Phoshporylated-Tau

Macrophage Subsets

3-plexed IHF Assay
CD68	CD163
empty/available space

Pancreatic Islet

3-plexed IHF Assay
Insulin	Ki-67
Secretogranin II

Pancreatic Islet

3-plexed IHF Assay
Insulin	BrdU
Pan-Islet Cell Marker

Custom build of multiplexed staining panels

Offspring offers custom design and construction of optimized IHF and IHC assays for simultaneous detection of 3-5 biomarkers in tissue sections, flexibly targeting your project-specific needs. We build these assays, using well selected fluorophors or chromogens and the highly sensitive, sequential TSA-based staining technique. The assays are established, optimized and validated to specifically measure each marker without cross-talk between each individual assay according to strict guidelines and criterias. These assays are designed to be run with high precision, consistency and efficacy on the Ventana Discovery Ultra platform.

Offspring also offers services for multiplexed staining of immune cells in tissues sections from human tissues, using kit reagents from:

Pre-established and flexibly adapted staining panels. 4-5-plexed IHF assays directed against various combinations of human immune cell markers. Hapten anti-hapten based detection technique

HI-1A	HI-2A	HI-3A	HI-4A	HI-5A	HI-6A
CD3	CD68	CD4	CD8	CD8	CD3
CD4	CD163	CD8	CD68	CD68	CD4
CD8	PD-L1	FoxP3	PD-L1	PD1	CD8
CD20	Pan-Cytokeratin	PD-1	Pan-Cytokeratin	PD-L1	FoxP3

Pre-established staining panels for analysis of various immune cell populations in human tissue sections. 4-plexed IHF assays. Sensitive rolling circle-based detection.

Panel I/O PD1	Panel I/O T-Reg	Panel I/O MDSC	Panel I/O PD-1	Panel I/O T-Act	Panel I/O APC
CD8	CD4	CD11b	CD3	CD3	CD11c
CD68	CD8	CD14	CD45RO	GrzB	CD20
PD-L1	FoxP3	CD15	PD-1	Ki-67	CD68/CD163
PanCytokeratin/ SOX10	PanCytokeratin	HLA-DR	PanCytokeratin /SOX10	PanCytokeratin/ SOX10	MHCII